中臺科技大學

Extraction and purification of plasmid DNA
Agarose gel electrophoresis 
Restriction enzyme digestion
Recovery and purification of DNA fragment on agarose gel
Phenol/chloroform extraction and alcohol precipitation
DNA ligation
Preparation and transformation of competent E. coli
Identification of bacterial colonies that contain recombinant plasmids
Polymerase chain reaction (PCR)

Bacteria culture

Extraction and purification of plasmid DNA

Gene cloning

Transformation

Identification of bacterial colonies that contain recombinant plasmids

SDS-PAGE

Western blotting

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